Substrates
High-Quality Substrates for Biochemical & Diagnostic Applications
DARIMOL Substrates from S P Pharma & Research are designed to support a broad range of biochemical, enzymatic, diagnostic and laboratory applications.
Our portfolio currently includes pNPP and Tris Salt, offering researchers and industry professionals reliable products with a focus on quality, consistency and dependable performance.
pNPP p-Nitrophenyl Phosphate
pNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used chromogenic substrate for alkaline phosphatase (ALP) activity assays. Enzymatic hydrolysis of pNPP produces the yellow-colored compound p-nitrophenol, allowing convenient colorimetric measurement of enzyme activity. The reaction is commonly monitored at approximately 405 nm.
pNPP is suitable for biochemical research, enzyme activity studies, diagnostic applications and ELISA-based assay systems.
Key Features
-
Chromogenic substrate for alkaline phosphatase
-
Suitable for enzyme activity assays
-
Produces a soluble yellow reaction product
-
Suitable for colorimetric detection
-
Useful for kinetic and endpoint determinations
-
Suitable for research and diagnostic applications
Our Substrates Products
Our Substrates Products
pNPP
p-Nitrophenyl Phosphate
pNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used chromogenic substrate for alkaline phosphatase (ALP) activity measurement. In the presence of alkaline phosphatase, pNPP produces a yellow, water-soluble reaction product that can be measured spectrophotometrically at approximately 405 nm.
Product Specification :
-
Chemical Name: p-Nitrophenyl Phosphate Disodium Salt
-
Molecular Weight: 263.05 g/mol
-
Molecular Formula: C₆H₄NNa₂O₆P
-
CAS No.: 4264-83-9
Product Features & Advantages:
-
Chromogenic substrate for alkaline phosphatase.
-
Suitable for colorimetric detection.
-
Useful for kinetic and endpoint determinations.
-
Suitable for research and diagnostic applications.
Product Specification :
-
Chemical Name: p-Nitrophenyl Phosphate Disodium Salt
-
Molecular Weight: 263.05 g/mol
-
Molecular Formula: C₆H₄NNa₂O₆P
-
CAS No.: 4264-83-9
Product Features & Advantages:
-
Chromogenic substrate for alkaline phosphatase.
-
Suitable for colorimetric detection.
-
Useful for kinetic and endpoint determinations.
-
Suitable for research and diagnostic applications.
Tris Salt
Tris(hydroxymethyl)aminomethane Hydrochloride
Tris Salt, commonly supplied as Tris Hydrochloride (Tris HCl), is a widely used biochemical buffering reagent. It is used to maintain controlled pH conditions in a variety of biochemical, molecular biology, enzyme research and laboratory applications. Tris HCl is commonly useful across a pH range around 7–9, with a pKa of approximately 8.1 at 25°C.
Product Specification :
-
Chemical Name: Tris(hydroxymethyl)aminomethane Hydrochloride
-
Molecular Weight: 157.60 g/mol
-
Molecular Formula: C₄H₁₁NO₃·HCl
-
CAS No.: 1185-53-1
Product Features & Advantages:
-
Chromogenic substrate for alkaline phosphatase.
-
Suitable for colorimetric detection.
-
Useful for kinetic and endpoint determinations.
-
Suitable for research and diagnostic applications.
Product Specification :
-
Chemical Name: Tris(hydroxymethyl)aminomethane Hydrochloride
-
Molecular Weight: 157.60 g/mol
-
Molecular Formula: C₄H₁₁NO₃·HCl
-
CAS No.: 1185-53-1
Product Features & Advantages:
-
Chromogenic substrate for alkaline phosphatase.
-
Suitable for colorimetric detection.
-
Useful for kinetic and endpoint determinations.
-
Suitable for research and diagnostic applications.
